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  • Evaluation of the adjuvant properties of plasmids coding for IFN-g or IL-12, of CpG immunostimulatory motifs (pUC18) and of the conventional adjuvants dimethyldioctadecylammonium bromide-in-water

Evaluation of the adjuvant properties of plasmids coding for IFN-g or IL-12, of CpG immunostimulatory motifs (pUC18) and of the conventional adjuvants dimethyldioctadecylammonium bromide-in-water

For PRV, early reduction of virus shedding and prevention of virus transmission is strongly related to the strength and the quality of cell-mediated immune responses. In addition to the search for immunorelevant antigens, ways to improve the efficacy of DNA vaccines were sought. We therefore evaluated the adjuvant properties of plasmids coding for IFN-g or IL-12, of CpG immunostimulatory motifs and of the conventional adjuvants dimethyldioctadecylammonium bromide-in-water (DDA) and sulfolipo-cyclodextrin in squalene-in-water (SL-CD). We demonstrated that a DNA vaccine combined with DDA but not with the other adjuvants, induced significantly stronger immune responses as compared to plasmid vaccination alone. Moreover, pigs vaccinated in the presence of DDA were protected against clinical disease and shed significantly less PRV after challenge infection.

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Netherlands
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