Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

A gelatine sponge implantation system for studying the local immune response

A sponge-implantation system was established for pigs to analyse ex vivo the local immune response after antigen application. To this end, gelatine sponges containing antigen or buffer as negative controls have been implanted subcutaneously, removed after vascularisation (day 5 post implantation), and subsequently phenotypes of the locally attracted T-lymphocytes were determined in two-colour flow-cytometry focusing on the activation markers CD25 and CD45RC. Comparative analyses were performed with matching PBMC derived from the same individuals.

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Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Institute of Immunology
Paul-Ehrlich-Str. 28
D-72076 Tubingen
Germany
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