Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

Production of recombinant proteins and new panel of SAV-specific monoclonal antibodies

To better characterize this new aquatic alphavirus and to make molecular tools available, a panel of monoclonal antibodies (Mabs) directed against SDV nonstructural and structural proteins has been generated by immunizing mice with SDV-specific recombinant proteins overexpressed in E. coli as antigens. So far, Mabs against nsP1, nsP3, E2 and E1 SDV proteins have been produced and their reactivity has been characterized by Western-blot, radio-immunoprecipitation and indirect immunofluorescence assays. In addition, protein domains recognized by each Mab have been determined by immunofluorescence assay on truncated expressed SDV-derived proteins. Finally, one Mab directed against the E1 glycoprotein has been evaluated as potential tool to be used in immunohistochemistry assay on experimentally infected trout.

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Unite VIM, INRA, CRJJ, Domaine de Vilvert
78352 Jouy en Josas
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