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Monoclonal antibodies to chlorophenols and chloroanisoles

The development of specific immunochemical methods that offer high sensitivity, high sample throughput whilst being low cost and simple to operate will aid traceability of toxic agents and hence reduce the level of toxic components within processed food products.

The applicability of this approach was assessed using toxic chlorophenolic fungicides and their chloroanisole breakdown products that were oxic contaminants of many food products at the part per billion levels. The present study focused on the development of monoclonal antibodies against penta-chlorophenol(PCP) and penta-chloroanisole (PCA) and its application to immunoassay procedures in the wine industry. The project was specifically target wine, fruit juice and potable water food production processes since the target compounds were commonly identified in such matrices due to fungicide treatment of raw materials.

Monoclonal antibodies were produced using established biological techniques at Diaclone. Chlorophenol (PCP) and chloroanisole (PCA) fungicide derivatives were conjugated to carrier proteins. Immunogens PCP-KLH and PCA-BSA were injected into mice. Monoclonal antibodies were obtained against PCP called B-L36 and B-P31 and against PCA named B-A46, B-G40 and B-R28. Using the mAb B-L36, an indirect competitive enzyme immunoassay (IEA) was developed for measurement of PCP. During preliminary studies, false positive diagnosis due to interference within the complex wine samples was obtained. To improve the correlation between gas chromatography (GC) and ELISA methods, the development of a highly efficient selective sample purification procedure to remove unrelated background matrix components has been reported.

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Research Dept
Diaclone, Bvd A. Fleming
25000 Besancon
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