Community Research and Development Information Service - CORDIS

New immunoassays, more specific and more sensitive for B. cereus enterotoxins

As new analytical tools monoclonal antibodies (mab) against the components of the NHE (and CytK)enterotoxins were developed which enable the specific and sensitive detection of these exoproteins in cell-free culture supernatants of Bacillus cereus isolates.

These mabs were successfully employed in a broad range of immunochemical techniques:
(a) by immunoaffinity chromatography highly pure toxin preparations could be obtained
(b) for the simple differentiation of HBL and/or NHE producing B. cereus isolates a colony immunoblot assay was established
(c) different EIA methods enabled the reliable detection of diarrhoeal B. cereus strains and their discrimination in high and low producers.

Particularly the developed sandwich enzyme immunoassays for the L2 component of HBL and the B component of NHE, respectively, represent a considerable improvement in sensitivity and specificity compared to the currently used assays. They permit a quantitative measure of the amount of NHE and HBL produced by a strain, thereby providing an estimate of the virulence of this strain with regard to diarrhoeal poisoning.

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Lehrstuhl für Hygiene und Technologie der Milch
8,Ludwig-Maximilian Universitaet Muenchen Schönleutner Str.
85764 Oberschleißheim
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