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Novel A. thaliana genes involved in the biosynthesis of antioxidants

Several thousand individual T-DNA activation-tagged lines from Arabidopsis containing an immobilised element (4x35S Enhancer, Basta Resistance, Inverted repeats) have been screened in co-operation with Partner 1 for high phenylpropanoid content by HPLC-Analysis using fluorescence and UV techniques. In the first round of screening, about ten mutant lines, which show modified HPLC profiles under greenhouse conditions have been defined as putants and selected for further studies. Three out of ten lines showing a high phenolics phenotype were studied in detail in a new set of recapitulation lines. Thus, accumulation of new chemoprotective antioxidants in mutant lines were determined by overexpression of genes with unknown functions - At1g18570, At2g20095 and At3g10820. Constructs for the expression of those genes in tomato plants have been created and sent to Partner 1.

Results have been published in A. Schneider, T. Kirch, T. Gigolashvili, H-P. Mock, U. Sonnewald, R. Simon, U-I. Flügge, W. Werr (2005) A Transposon-Based Activation-Tagging Population in Arabidopsis thaliana (TAMARA) and its Application in the Identification of Dominant Developmental and Metabolic Mutations. FEBS Lett. 29;579(21):4622-8.

Reported by

UNIVERSITAET KOELN
Gyrhofstrasse 15
50931 KOELN
Germany
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