Community Research and Development Information Service - CORDIS

Microarray technology

Most blood group alloantigens specific for red cells and platelets are based on single nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. Using five Human Platelet Antigen (HPA) systems as a model a 4th generation microarray technique for SNP typing was established by CR4. In parallel, a 3rd generation technique, the TaqMana assay, was developed by CR2. Both techniques were validated by using a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and -15 systems. Unambiguous and concordant results were obtained with both techniques for all samples. The data validate the use of microarray for large scale SNP typing for clinically relevant blood group alloantigens.

Reported by

Universität Heidelberg- DRK - BLUTSPENDEDIENST BADEN-WUERTTEMBERG - Institue of Transfusion Medecine and Clinical Immunology - Friedrich-Ebert-Strasse 107
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