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RECOMBINANT BCG Résumé de rapport

Project ID: ICA4-CT-2000-30032
Financé au titre de: FP5-INCO 2
Pays: Brazil

stability of rBCG vectors

Prior to testing candidate vaccines in cattle it is necessary to characterise the rBCG strains in terms of their ability to survive in vivo, express recombinant antigen and stimulate an immune response. In the context of the current project it was considered essential to develop a model system with which to rapidly, reproducibly and meaningfully assess the behaviour of candidate rBCG constructs in the bovine intracellular environment. A method was established for the use of cultured bovine PBMCs so that rBCG vaccine candidate constructs most likely to function in cattle could be selected.

Reported by

Instituto de Tecnologia em Imunobiológicos da Fundação Oswaldo Cruz
Laboratorio de Tecnologia Recombinante, Pavilhão Rocha Lima, sala 411, Av. Brasil, 4365 - Manguinhos
21045900 Rio de Janeiro