Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

RECOMBINANT BCG Résumé de rapport

Project ID: ICA4-CT-2000-30032
Financé au titre de: FP5-INCO 2
Pays: United Kingdom

generation of leuD KO rBCG strains

I recombinant BCG strain was generated, rBCG-leuD, in which the leuD gene was deleted. The strain was therefore an auxotroph for the amino acid leucine. This allowed the use of plasmid vectors that provide the leuD gene in trans that complement the leuD mutation. This complementation allows the stable expression of foreign genes in BCG in the absence of antibiotic resistance markers.

Reported by

UNIVERSITY OF SURREY
Guildford
GU2 7XH GUILDFORD
United Kingdom
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