Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Novel Pre-Immobilised Enzymes for Sensing Applications

The encapsulation of enzymes by trapping during polymerisation using different compounds may be quite complicated when using very reactive or hydrophobic reagents, because they can directly inactivate the enzyme or reduce their stability by generation of non-favourable environments considering enzyme stability. Our group proposes the pre-immobilization of the enzymes on pre-existing porous micro-particles, mainly if the polymer is extremely hydrophobic as in the case of ORMOCER. In this way, the stability of the enzyme will be determined by the properties of the support used in the immobilization and the techniques and know-how from our lab can be directly applied to this new problem, while the enzyme will not be in contact with the polymer, decreasing the requirement imposed to it. Moreover, because these beads present some rigidity, the percentage of biocatalyst percentage weight may be higher that using soluble enzyme. This way, the higher activity recovery, the higher stability and the higher amount of biocatalyst may greatly improve the biosensor performance.

Moreover, new purirification techniques will be developed to the industrial purification of the target enzymes.

Reported by

Instituto de catalisis. C/ Marie Curie. CAmpus UAM Cantoblanco
28049 MADRID
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