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Genomics of carotenoid biosynthesis in Blakeslea

The formation of b-carotene is highly induced when the two mating types of B. trispora mate. Molecular genetic analysis of carotenogenesis in Blakeslea trispora is being studied using cDNA AFLP transcript profiling technique. The cDNA AFLP technology has been set up in order to identify genes that are involved in this process. One of the important steps in this technique is the direct determination of the nucleotide sequence from the AFLP patterns. The common technique was modified to give good result for fungal gene expression. Numerous (fragments of) genes are in the process of being identified and their sequnce analysis and gene identification of genes that are potentially involved in the induced carotenogenesis process of B. trispora using bioinformatics techniques is under way.

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Dreijenlaan 2 P.B. 9101
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