Wspólnotowy Serwis Informacyjny Badan i Rozwoju - CORDIS

Analysis of polyamines and aromatic amines of cells and tissues to determine the effect of preculture, desiccation, freezing and application

Polyamines are polycations and thus bind readily to important cellular polyanions like DNA, RNA, phospholipids and acidic protein residues and cell wall components. There are more and more indications that polyamine metabolism is also strongly involved in the plant response towards environmental challenges such as osmotic stress, salinity, hypoxia, cold stress and environmental pollutants.

Analysis of polyamines and aromatic amines is a best way to understanding possible mechanisms involved in the cryo-protecting process. Sugar (particularly sucrose) pretreatment is essential to survive cryopreservation for most of plant species. Result 5 focused on the change in polyamines and aromatic amines induced by sugar / sucrose pretreatment. The knowledge is implemented to improve the existing cryopreservation protocols for diverse plant species ensuring wide application potential.

Key results:
- Previously unknown aromatic amines were identified;
- Sugar / sucrose pretreatment resulted in change in polyamines and aromatic amines;
- Such change is closely associated with cryopreservation ability;
- The polyamine level could be comparable to the control during the post thaw regrowth
- Application of specific polyamines or aromatic amines during the preculture process could improve post thaw regeneration after cryopreservation.

This achievement is not only helpful for improve the conservation of plant germplasm but also provides valuable information with respect to possible mechanisms of abiotic stress (especially drought and frost) resistance in plants.

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Reported by

Katholieke Universiteit Leuven
Laboratory of Tropical Crop Improvement, Kasteelpark Arenberg 13
3001 Heverlee
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