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Analytical techniques to determine cytoskeletal protein modification/loss and cell signalling pathways for the use as markers of desiccation and cryotolerance

Assessment of the extent of modifications/loss of proteins associated with the cytoskeleton and signalling pathways is a powerful means of pinpointing critical injurious points of cryo-conservation protocols; application of which is used to improve cryo- methods.

Result 6 is the development of transglutaminase assays and identification of markers of desiccation and freezing tolerance for profiling cryoinjury; the result can be implemented to improve and design new cryo-conservation protocols for diverse species ensuring wide application potential.

Critical success factors:
- Formulation of storage solutions based on informed knowledge of cryoinjury rather than empirical know-how.

- Increased efficiency of storage development through identification of critical points using markers of protein modification/loss. Hence minimising germplasm sacrificed in cryostorage work up

- Enhanced confidence in cryostorage by improved amelioration of cell injury.

Innovative benefits: bespoke techniques for application by end users: germplasm repositories/coillections, , botanic gardens and biotechnology companies. Helping to meet their needs to hold germplasm in cryogenic storage as the only long-term means of securing non-orthodox germplasm ex situ. The major strength of the result is the integration of technological and fundamental approaches to provide markers of cell injury that assist the design less damaging cryostorage protocols, particularly for storage recalcitrant germplasm.

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University of Derby
Biological Sciences, School of Education Health and Sciences, Kedleston Road
DE22 1GB Derby
United Kingdom
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