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Analytical techniques to monitor oxidative stress in cryopreserved plant germplasm, and their application in the development of new and improved storage strategies

Monitoring oxidative damage is a powerful means of pinpointing components of storage protocols that cause injury, application of this fundamental knowledge is used to improve cryo-conservation methods.

Result 7 is an analytical toolbox for profiling oxidative processes in cryopreserved germplasm the result is implemented to improve and design new cryo-conservation protocols for diverse species ensuring wide application potential.

Critical success factors:
- Formulation of storage solutions based an informed knowledge of cryoinjury rather than empirical now-how.
- Increased efficiency of storage development through identification of critically injurious points using generic oxidative stress markers.
- Implicit to minimisation of germplasm sacrificed in cryostorage work up.
- Enhanced confidence in cryostorage due to enhanced amelioration of stress.

Innovative benefits: bespoke techniques for application by end user practitioners: germplasm repositories, seed banks, culture collections, botanic gardens and biotechnology companies. Helping to meet their needs to hold germplasm in cryogenic storage provides the only long-term means of securing non-orthodox germplasm ex situ. The major strength of the result is the integration of technological and fundamental approaches to provide generic stress markers that assist end users to design less damaging cryostorage protocols, particularly for difficult to conserve, germplasm that hitherto is storage recalcitrant.

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Independent
Drum Road, Cuparmuir, Cupar
KY15 5RJ FIFE
United Kingdom
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