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Protocol for the transient, dsRNA based silencing of putative pathogenicity factors from cyst nematodes

RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described.

Here, we use a modified method to analyze the function of secreted â-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plantparasitic nematode. Knockout of the â-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.

Reported by

Scottish Crop Research Institute (SCRI)
DD2 5DA Dundee
United Kingdom
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