Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Identification of a polygalacturonase (PG) from infective juveniles of root knot nematodes - the first PG of animal origin

The ¢rst animal polygalacturonase (PG, EC 2.1.15) encoding cDNA, Mi-pg-1, was clonedfrom the plant parasitic nematode Meloidogyne incognita. The enzymatic activity of MI-PG-1 was con¢rmedafter heterologous expression in Escherichia coli. The presence of a predicted signal peptide on the MI-PG-1 sequence together with the speci¢c localization of the transcripts of the Mi-pg-1 gene in the oesophageal glands of infective juveniles imply that MI-PG-1 couldbe secretedinto plant tissues. The potential role of MI-PG-1 in parasitism is discussed.

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INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
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