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Definition of gene expression signatures of effects of Endocrine Disruptors in testis development. Molecular markers. Designed of specific DNA arrays

An Endocrine Disruptor (ED) is an exogenous substance that causes adverse health effect in an intact organism, or its progeny, secondary to changes in endocrine functions. The reproductive systems have been considered a crucial target of EDs generating gene deregulation. Consequently, the analysis of the genetic effects of EDs on developing gonads has repercussions on the effects upon the individuals and the progeny.

By gene-expression microarrays, the effects of four different EDs: lindane, bisphenol-A, monoester of ethylhexyl phthalate and zearalenone and estradiol, upon testis development were investigated.

Three different dosages for each compound were supplied with the drinking water to mice during three precise periods of development:
- To the mothers before mating;
- Same dosages maintained during pregnancy; and
- Maintained four weeks after birth.

The mouse transcriptome were analysed. Comparative analysis of all data defined gene expression signatures. Most of the EDs analysed in this study have a distinctive pattern of gene deregulation from the Estradiol. Phthalate and zearalenone showed defined pattern. After validation of data obtained from microarray analysis, selection of specific genes can be processed to build a specific GENDISRUPT microarray that could be patented and used in the analysis in animal models of suspected EDs.

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Reported by

Centro de Investigaciones Biologicas. C.S.I.C.
Ramiro de Maeztu, 9
28040 Madrid
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