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Biochemical markers for oxidation

Abiotci stress causes increased production of activated oxygen species able to inactivate enzymes and damage cellular components. H2O2 functions in a concentration-dependent manner - as a signal at low concentrations and phytotoxically at higher. MDA an end product of lipid peroxidation is used extensively as indicator for membrane injuries and production of free radicals.Short-term salt stress in vitro led to decrease of H2O2 in tolerant P. euphratica and P. alba 2AS poplars.

Longer stress resulted in H2O2 increase in sensitive P. tremula x tremuloides, P. alba x tremula and P. alba 6K3. H2O2 levels continued to be significantly lower in tolerant clones.Sensitive poplars showed increased MDA levels while the tolerant were with significantly reduced MDA.

Under salt stress in pots similar patterns of H2O2 were found in poplars on normal and salty soils. P. euphratica showed particularly low levels of H2O2. Salt sensitive genotypes were with significantly higher MDA levels during stress. No differences between control and stressed tolerant plants were found.

In our experiments, the salt tolerance of P. euphratica and P. alba 2AS appears to be related to maintenance of H2O2 and MDA levels similar or lower to those under normal conditions.

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Reported by

AgroBio Institute
8 Dragan Tzankov Blvd.
1164 Sofia