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AFLP characterisation of Aspergillus Section Nigri

The data of AFLP analysis performed on 300 strains with the primer A showed a clear pattern’s peaks for the three species main occurring on grapes. So we are able to construct a dendrogram of similarity that evidenced the grouping in three distinct clusters of the strains belonging to A. carbonarius, A. niger group and A. japonicus/A. aculeatus .

These result were successively confirmed by the AFLP analyses made with other three primers (B, C and D), all the results obtained gave good fingerprints useful to construct dendrograms that clearly separate the three main groups of the Black Aspergilli (Fig. 5).

The AFLP banding pattern was variable from 20-25 bands for A. carbonarius and A. japonicus aggregate to 30-40 bands for A. niger aggregate, confirming the higher genetic variability among this species aggregate as was showed by sequence analyses.

On the basis of these great variability found in the A. niger aggregate strains the AFLP analysis revealed the presence of two main clusters within this species aggregate that seem clearly separated, suggesting the possible split in two different species of this two groups of A. niger strains.

Reported by

National Research council of Italy
Via Amendola 122/O
70126 Bari
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