Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS


ASIARESIST Informe resumido

Project ID: ICA4-CT-2001-10028
Financiado con arreglo a: FP5-INCO 2
País: Belgium

SOP-ASIARESIST-PRES, for preservation of bacterial isolates

A Standard Operating Procedure (SOP) document was defined for the preservation of isolates that were obtained in the course of the project. The use of a commercial cryobeads system was proposed to store strains for a prolonged period as defined in SOP-ASIARESIST-PRES.

The final version of the SOP was obtained following intensive discussions among partners and was made available to all partners during the lifetime of the project via the restricted access area of the project website. To validate this SOP, two harmonization strain sets were send to all partners that participated in the sampling and sample processing activities.

The partners were asked to
- subculture the strain set,
- prepare cryovials for long-term preservation, and
- send subcultures recovered from cryopreservation to Partner 2.

The strains in the first set consisted of organisms that represented different bacterial groups known to be (pre)dominant in aquaculture environments i.e. Aeromonas hydrophila, Vibrio harveyi, Stenotrophomonas maltophilia, Salmonella enteritidis, Escherichia coli and Acinetobacter junii. A second harmonization strain set was send to represent organisms that are considered to be involved in aquaculture infections including Aeromonas salmonicida, Photobacterium damsela, Yersinia ruckeri, Vibrio mediterranei and Vibrio alginolyticus.

These control strains were stored using the cryobeads system in each partner country and subsequently checked for recovery and send back to Ghent University for confirmation of the taxonomic identity with fatty acid analysis. No major inconsistencies were noted in this regard, thus indicating that the document is a valuable guide towards the global preservation of bacteriological material in geographically separated labs.

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