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ASIARESIST Report Summary

Project ID: ICA4-CT-2001-10028
Funded under: FP5-INCO 2
Country: Belgium

SOP-ASIARESIST-SAMPLE, for sampling aquaculture environments

A Standard Operating Procedure (SOP) was defined for sampling and sample processing of aquaculture environments (SOP-ASIARESIST-SAMPLE). This SOP was developed based on the decision to examine chloramphenicol (CHL) resistance.

The final version of the SOP was made available to all partners via the project website. A critical step in this matter concerned the decision on the breakpoint concentration, i.e. the concentration CHL to be added to the isolation medium for selection of acquired resistances rather than low-level intrinsic resistances. Following experimental work by SEA partners and taking into account literature data, it was decided to set the breakpoint concentration at 35 ppm.

A consensus version of SOP-ASIARESIST-SAMPLE was formulated in which clear indications are given on the number of sampling points that need to be identified in Thailand, Malaysia, and Vietnam, the choice of the sampling equipment for water, sediment, and organism samples, the number of isolates that should be generated per site and per country, and the strain designation code to be used as a key to enter data in the project database.

Reported by

K.L. Ledeganckstraat 35
9000 GENT
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