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ASIARESIST Résumé de rapport

Project ID: ICA4-CT-2001-10028
Financé au titre de: FP5-INCO 2
Pays: United Kingdom

PCR detection of cat1-3 genes

A multiplex PCR assay was evaluated for the PCR detection of cat1-3 genes (Yoo et al., 2003) among the chloramphenicol resistant strain collection. Primers specific to the three distinct cat genes are combined with a single conserved primer. The individual cat genes are distinguished by virtue of size differences in the PCR products.

The presence of cat1 and cat2 PCR products was confirmed by sequencing. No cat3 genes were detected among the strain collection. In the rare instances where more than one cat gene was present within an isolate, the application of a single specific primer was found to be beneficial for clarification purposes. The method is recommended as a screening method for the detection of cat1-3 genes.

Reported by

University of Stirling
Institute of Aquaculture, Pathfoot Building, University of Stirling
FK9 4LA Stirling
United Kingdom
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