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FP5

ASIARESIST Report Summary

Project ID: ICA4-CT-2001-10028
Funded under: FP5-INCO 2
Country: United Kingdom

Biochemical assay for CAT activity

A biochemical CAT assay was optimised for the detection of CAT activity from aquaculture-associated genera. The method was adapted to a microtitre plate format to minimise cost. A change in colour from colourless to bright yellow indicated CAT activity. A strategy to confirm suspected CAT negative reactions was proposed. The assay was used to screen for the presence of CAT activity in those project isolates without known cat1 or cat2 genes.

Reported by

University of Stirling
Institute of Aquaculture, Pathfoot Building, University of Stirling
FK9 4LA Stirling
United Kingdom
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