Wspólnotowy Serwis Informacyjny Badan i Rozwoju - CORDIS

Demonstration of the functional response of olfactory receptors on gold electrodes and microelectrodes by electrochemical impedance spectroscopy

The effect of specific odorant (heptanal and octanal) and non-specific odorant (helional) on the electrical properties of the biofilm formed by the self-assembled monolayer, the antibody and the membrane fraction including the olfactory receptor has been shown by electrochemical impedance spectroscopy. Despite of non-specific effects for concentration of odorant higher than 10E-9M, specific recognition appears clearly with concentrations of odorant between 10E-13M and 10E-9M.

Many test controls were performed with self-assembled monolayer including the single antibody, including the specific antibody and rhodopsin and the specific antibody and olfactory receptor ORI7. The effect of solvent DMSO is less than 10%.

In such tests it clearly appears a higher specificity of ORI7 for heptanal and for octanal.

Cmyc-OR 1740 was anchored on the mixed SAM using the monoclonal anti-cmyc antibody. The specificity of OR 1740 for helional, compared to heptanal has been studied. An optimum sensitivity is observed for a concentration of 10-10M of helional. It has been checked that without any OR, the mixed SAM presents no variation of polarisation resistance in presence of helional, whatever its concentration.

The effect of the presence of GTP on the sensitivity of detection has been studied by the same technique. The presence of GTP increases the sensitivity of detection by a factor of 6 due to an amplification effect already observed by SPR measurements.

This result constitute one of the main results of the project and prove one of the hyphothesys on which it was based, namely, that biosensors based on the electrical response of olfactory receptors can be developed.

Reported by

Marti i Franques, 1
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