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A DON induced wheat cDNA library in a phage lambda vector allowing excision of yeast expression vectors

We have gnerated a cDNA library from wheat, which was treated with deoxynivalenol. The primary library is a phage lambda library in vector lambda PG5 (Brunelli and Pall, 1993) with a complexity of 2.10E6pfu. From the amplified library we have generated plasmid DNA pools by Cre/lox mediated in vivo excision (more than 50% have inserts between 750 and 1500bp.

The library can be used as phage library for cDNA screening or the excised plasmid to transform yeast (the cDNA inserts are expressed under control of the PGK1 promoter).

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Institute of Applied Genetics and Cell Biology,
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