Wspólnotowy Serwis Informacyjny Badan i Rozwoju - CORDIS

Improved inoculation techniques for Saatzucht Donau

A list of 111 winter wheat varieties and lines which were described as moderately to highly resistant (together with few susceptible checks) has been characterized for Fusarium head blight in replicated experiments for 3 years. The lines were tested using artificial inoculation with F. culmorum strains produced by P1 by spraying directly on the heads during anthesis. To insure enough humidity the application time was in the evening or just before proposed rainfall. Since our location is situated beside a river no further humidity control was necessary.

he inoculum concentration was 500.000 conidia/ml and application was done twice within one week for each plot. Evaluation of FHB was assessed in % diseased spikelets/ear( = severity) on a linear 0 - 100% scale 3- 4 times, beginning 10 days after inoculation. The scores from each year were calculated as AUDPC values (=area under disease progressive curve) to be able to compare years and locations. Highly significant differences between FHB resistance of the tested lines could be detected by variance analysis. Also a high accuracy and repeatability of our data can be seen, which has been calculated on basis of the intensive phenotyping by all involved project partners. A seed set of genotypes out of this screening nursery (excluding restricted breeding lines) is available for any interested breeding company. Resistance data of the whole set will be published by our consortium.

Hence any interested breeder has the possibility to compare and improve his artificial inoculation technique regarding the evaluated resistance level of the analysed genotypes from our consortium. The improved inoculation method is an efficient and reliable tool for us to select new genotypes within our wheat breeding program for FHB resistance to develop varieties with low mycotoxin content. Out of the most resistant lines from the nursery we already started a crossing program to develop more adapted resistance sources and finally new varieties.

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