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RFLP characterisation of Aspergillus niger aggregate

In order to characterise by molecular methods the Aspergillus niger aggregate species involved in the ochratoxin A (OTA) contamination of European wine grapes and table grapes from Israel, a total of 173 strains were studied. The ITS-5.8S rDNA fragments of 173 A. niger agreggate strains from grapes included in this study were amplified and their PCR amplicons were RsaI digested in order to classify the strains in the RFLP types, N and T. This technique was previously defined in our laboratory. All of the strains belonging to the A. niger aggregate were classified into the two RFLP types previously defined: type N (43%) and type T (57%). Twenty out of the 173 strains of A. niger aggregate produced OTA (0.1 to 10.5 mg g-1). All the OTA producing species belonged to the N-RFLP type. This RFLP characterization has been proposed as a technique for screening possible OTA producing A. niger aggregate strains due to the fact that all the OTA-producing isolates whose RFLP pattern is known have been classified as type N, whereas none of the type T strains are able, at the moment, to produce this mycotoxin.

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Univertitat Autonoma de Barcelona
Campus de la UAB Bellaterra
08193 Barcellona
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