Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

Development of rapid screening assays based on immunochemistry and time-resolved fluorometry

Council Directive 96/23/EC requires residue monitoring from the NRLs. There is strong evidence that coccidiostat residues can occur in poultry products, and consumers may be exposed to harmful residue concentrations. However, the lack of suitable methods has complicated the mandatory residue control.

To address this issue, rapid immunoassays were developed for the screening of three coccidiostats, halofuginone, nicarbazin and nitroimidazoles. The assays were based on the use of highly specific polyclonal antibodies, time-resolved fluorometry and a novel all-in-one dry chemistry assay concept. The dry chemistry concept enabled a one-step assay procedure, which could be fully automated with the help of an immunoanalyser.

All the assay specific reagents were predispensed into a single microtiter wells in advance and dried. Only the addition of a sample was required to start the assay, and the total assay time was 18 minutes. The sample material consisted of poultry eggs and liver. Prior to applying the samples to immunoassays, the samples were manually extracted according to a simple protocol. All the screening assays were validated according to Commission Decision 2002/657/EC.

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Reported by

University of Turku, Department of Biotechnology
Tykistökatu 6A
20520 Turku
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