Community Research and Development Information Service - CORDIS

Production of high-quality test kits for the screening of halofuginone, nicarbazin and nitroimidazoles

High quality test kits were produced for coccidiostats halofuginone, nicarbazin and nitroimidazoles. The all-in-one dry chemistry microtiter wells were coated with anti-rabbit IgG and then the analyte specific antibody was immobilised to the well. The insulation layer was dispensed into the wells and dried. The label was added in a small volume on top of the layer and dried. A custom-built small-volume dispensing machine was used for label dispensing during the assay development stage, when only few test kits were produced at a time.

However, the scaling up of the process proved to be difficult. Lot of effort was put into the tuning of the system, but the results were not satisfactory. Therefore, a completely new system for small volume label dispensing was constructed based on different kind of dispensing technique. However, the system could not be completed in time. Consequently, in the production of the test kits the label was dispensed manually to the microtiter wells. The stability and accuracy of the test kits were monitored during a one-year period. In addition, accelerated stability tests were conducted and the results were in accordance with the real time stability tests indicating that the kits were stable at least a year.

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Reported by

University of Turku, Department of Biotechnology
Tykistökatu 6A
20520 Turku
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