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Cell culture protocol for insect neurons

For the integration of in vitro networks of insect neurons on uncoated, silicon oxide extracellular recording sites we required a cell culture protocol that allows a low density, long-term stable neuronal cell culture of Gryllus bimaculatus and Locusta migratoria. Retaining of neurites and electrophysiological activity were required to assure the functionality of reconstructed neural networks.

A high quality cell culture protocol adapted from methods described in literature was established for cricket (Gryllus bimaculatus) as well as for locust neurons (Locusta migratoria).

Reported by

FORSCHUNGSZENTRUM JULICH, INST. BIO- & NANOSYSTEMS
LEO-BRANDT-STR.
52425 JULICH
Germany
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