Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

Method for preparation of cell free Human Herpes Virus-8 (HHV-8) inoculum

A novel system for producing HHV-8 cell free virus inoculum was established. This was obtained from activated BC3 cells and subsequent purification and concentration by high-speed centrifugation.

The second step was in identifying a continuous cell line supporting viral production. The experiments showed that Vero cells are permissive to HHV-8 replication, and efficiency of virus production is similar (even higher) than that of primary endothelial cells, a well-recognized cell target of HHV-8 infection.

We are trying to enhance the system, by production of Vero cell clones stably transformed with ORF50, a key promoter of HHV-8 replication.

Verwandte Informationen

Reported by

Section of Microbiology
via Luigi Borsari 46, 44100 Ferrara, Italia
44100 Ferrara
See on map
Folgen Sie uns auf: RSS Facebook Twitter YouTube Verwaltet vom Amt für Veröffentlichungen der EU Nach oben