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Set up and characterization of cell systems supporting Human Herpes Virus-8 (HHV-8) infection

It was determined that several cell lines are susceptible and permissive to exogenous HHV-8 infection.

The cell types suitable to conduct HHV-8 in vitro infection include: human endothelial cells from umbilical cord (HUVEC, a primary cell culture representing one of the natural targets of in vivo HHV-8 infection), Vero (a continuous cell line derived from monkey kidney and permissive for several human viruses), 293 (a human kidney epithelial derived continuous cell line), ECV-304 (a human cell line presenting markers specific of endothalial cells), U937 (a monocytic cell line) and primary human macrophages from healthy blood donors.

HHV-8 did not induce any lytic cytopathic effect, but in HUVEC cells induced a typical spindle shape phenotype. All cell lines supported lytic replication, as shown by analysis of viral transcription, and by release on infectious virus in culture supernatant.

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Section of Microbiology
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