Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Establishment of a protocol for induction of upper-airway inflammation in mouse (mouse model of allergy)

A mouse model of allergy to Ovalbumin for studying the inflammatory immune response has been established in collaboration with Dr. Sarah Howie (University of Edinburgh).

The protocol we have followed implies 2 intra-peritoneal administrations of alum-precipitated-Ovalbumin at weekly intervals, followed by 3 intra-tracheal (i.t.) injections of soluble Ovalbumin at 4 day intervals and sacrifice of mouse with collection of blood sample/ organs/ BALF at different time points after the last i.t. administration of antigen.

Reported by

Consiglio Nazionale Ricerche
Istituto di Genetica A. Buzzati Traverso, Via P. Castellino, 111
75010 Napoli