Community Research and Development Information Service - CORDIS

pCMVHuIC80Derp1 plasmid containing the gene encoding for the dust mite allergen of Dermatophagoides pteridissinus (DerP1) fused to aa 1-80 of human MH

Plasmid pCMVHuIC80Derp1 is an in-house developed construct containing the Der P1 gene, to be used for cell transfection experiments. In this plasmid, DerP1 is cloned as fusion to the leader sequence of the human MHC class II invariant chain, in order to target Derp1 expression into MHC class II pathway.

The plasmid is able to express the encoded antigen in vitro transiently transfected cells. This construct is particularly suitable for in vitro studies aimed at analysing the antigen processing and presentation by human APCs, and to evaluate cell activation upon DNA uptake. The application can be disseminated as further R&D.

Reported by

Novartis Vaccines and Diagnostics Srl (ex Chiron)
Via Fiorentina, 1
75010 Siena
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