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Procedure for preparation of molecularly imprinted polymer films based on spin coating

Molecularly imprinted polymer films by spin-coating.

This item constitutes a robust technique for preparing molecularly imprinted polymer (MIP) films, with good control of thickness and porosity. The use of polymer porogens facilitated access to the synthetic receptor sites, and the relationship between the morphology and binding properties was rigorously investigated using AFM and autoradiography.

Spin-coating is one of the standard coating techniques used for example in the microelectronics industry. We have recently demonstrated that it is possible to spin-coat the monomer mixture on a flat surface followed by in-situ photopolymerization in the presence of a template, in order to obtain MIP films with thicknesses between 100 nm and several µm.

However, with acrylic and vinyl monomers, polymerization of these films is too fast for phase separation to occur, so that non-porous films with very little binding capacity is obtained. However, it is possible to accelerate phase separation by adding a linear polymer such as poly(vinyl acetate) as co-porogen.

The porosity of the films can be fine-tuned via the amount and the molecular weight of the poly(vinyl acetate), and even surfaces covered with nanoparticles can be obtained (Figure 5). We found that in contrast to bulk polymers, in these systems phase separation and pore formation is by spinodal decomposition.

These MIP films can be used as selective recognition layers in biosensors.

Reported by

ARC Seibersdorf research GmbH
Forschungszentrum Seibersdorf
2444 Seibersdorf
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