Wspólnotowy Serwis Informacyjny Badan i Rozwoju - CORDIS

Development of functional eukaryotic expression plasmids encoding core and envelope genes of MVV for use as immunogens

The p55gag and gp150env precursor genes, which encode the core and envelope proteins of maedi visna virus have been cloned and ligated into a eukaryotic plasmid expression vector that expresses the gag and env recombinant proteins in mammalian cells both in vitro and in vivo. These plasmid vectors can be used as immunogens in vaccine studies (as in this project) using plasmid DNA vaccination methods.

The vectors have other potential uses. They could also be used to study the gag and env precursor virus proteins in terms of their basic structure, their interaction with cellular proteins, their processing into individual virus proteins (i.e. p25, p17, p14 core proteins from the gag precursor; and gp135, gp46 envelope proteins from the env precursor), and their use as diagnostic reagents. The env precursor gene from MVV has not been expressed from a plasmid construct to date and this represents an innovative successful element of the project.

The end users of the information are other scientists working in the field of SRLV control, or working with other lentiviruses, or working with other plasmid DNA vaccines, or in the general area of vaccines and immune responses, or in the general area of lentivirology.

Commercial companies and organisations concerned with SRLV control or regulation may be interested in the plasmid reagents. However, the market for these is likely to be restricted to the SRLV research community and therefore very small. These end users are unlikely to be involved until there is a usable effective vaccine in place.

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