Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Development of whole virus ELISA to detect and measure the level of antibodies to MVV in blood

MVV EV1 was grown in tissue culture and purified by sucrose density centrifugation. The purified EV1 was used as antigen to develop a whole virus ELISA to detect and quantify antibodies to MVV in serum samples. The ELISA can be used to detect seroconversion and antibody titre in experimental animals (as in this project) or to detect antibodies for routine diagnostic purposes.

The end users of the information are other scientists working in the field of SRLV control, or working with other lentiviruses, or working with other plasmid DNA vaccines, or in the general area of vaccines and immune responses, or in the general area of lentivirology.

Commercial companies may be interested in the whole virus ELISA, but a whole virus ELISA is available commercially already and the ELISA developed within the project has no particular advantage in terms of sensitivity or specificity over the commercial ELISA. Since there are 4 commercially available ELISAs for SRLV diagnosis the potential market is small and it is therefore unlikely that the project ELISA would be commercialised.

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