Community Research and Development Information Service - CORDIS

Development of real-time PCR method to detect and quantify ovine IFNg and IL4

Real-time PCR tests to detect and quantify ovine IFNg and IL4 mRNA in peripheral blood mononuclear cells were developed. The primers were based on published sequences and represent pre-existing knowledge. The real-time PCR tests can be used to detect and quantify mRNA specific for IFNg and IL4 in peripheral blood cells in experimental animals involved in vaccine studies (as in this project) or in pathogenesis studies. The tests could be used as diagnostic reagents in routine diagnosis of SRLV or other pathogens. This would be performed by measuring production of IFNg or IL4 mRNA by T cells after stimulation with antigens specific to the pathogen (MVV EV1 in this project, but any suitable antigen in the case of other pathogens).

The end users of the information are other scientists working in the field of SRLV control, or working with other lentiviruses, or working with other plasmid DNA vaccines, or in the general area of vaccines and immune responses, or in the general area of lentivirology, or in the general area of animal disease.

Commercial companies may be interested in the IFNg or IL4 real-time PCR tests, but several similar assays to detect bovine IFN? or IL4 mRNA have been published. The market for such assays is likely to be small unless high throughput modifications were developed which allowed the assays to be performed on large numbers of samples. Further work is required to determine if the assays have any value in routine diagnosis.

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