Wspólnotowy Serwis Informacyjny Badan i Rozwoju - CORDIS

Generation of recombinant MHC class 1 and b-2 microgobulin from inbred swine an and methods for identifying peptides bound to porcine MHC class 1 proteins

Cytotoxic T-cells (CTL) provides a key arm of the cellular immune response to viruses. CTLs kill cells infected with viruses and deprive viruses of a site of replication. CTL recognition takes place at the surface of cells and involves recognition of MHC class 1 proteins containing peptides derived from a viral proteins.

An understanding of the nature of peptides bound to MHC class 1 and the viral peptides recognised by CTLs is required for the rational design of vaccines effective against viruses. This project has developed tools to study the natures of peptides bound to porcine MHC class 1 proteins and started to apply this knowledge to a study of proteins encoded by CSFV. Importantly, the genes for pig MHC class 1 proteins have been cloned and sequenced from two lines of inbred pig commonly used to study immune responses to porcine viruses and bioinformatic analysis has identified putative peptide binding grooves.

The genes have been subcloned into vectors that allow recombinant MHC protein to be produced in bacteria and then purified by affinity chromatography. The proteins are now available for refolding studies using overlapping 9mer peptides derived from CSFV in particular, and pig viruses in general. This work paves the way for the design of high through put "in vitor" assays that can identify peptides that bind preferentially to pig MHC class 1 protein within complex pools of peptides.

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United Kingdom
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