Community Research and Development Information Service - CORDIS

Determination of potato cyst nematodes by enzyme linked immuno sorbent assay

Potato cyst nematodes Globodera pallida and G rostochiensis, cause substantial losses of potato production each year. An accurate, rapid identification method is proposed which will utilize monoclonal antibodies that will differentially recognize the two species of potato cyst nematodes (PCN) and which can be used in an enzyme linked immunosorbent assay (ELISA) to quantify population densities of PCN.

Nematode populations and potato genotypes appropriate for selection of virulence have been assembled and candidate propagation systems have been compared. Conditions for using a closed container system have been overcome by appropriate sterilization procedures. Polyclonal antisera and monoclonal antibodies (MAbs) have been raised to the 2 species of potato cyst nematodes (PCN). 2 polyclonal antisera raised against juvenile homogenates are being used experimentally. So far, 3 fusions to give cell lines producing MAbs raised against juvenile homogenates or juvenile proteins have been performed. About 20 antibodies which recognise the 2 species of PCN differentially have so far been identified and 2 of these have been used successfully in quantification assays.

Reported by

ROTHAMSTED RESEARCH LIMITED
West Common
AL5 2JQ Harpenden, Herts
United Kingdom
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