Community Research and Development Information Service - CORDIS

Enteric viruses for preventing water-borne diseases

Animal residues constitute a key source of viral environmental pollution and impose a great hazard for human health. Aided by biotechnology, a recently developed Spanish technology offers viral identification of animal residues in environmental samples, including water.
Enteric viruses for preventing water-borne diseases
The Reverse Transcriptase-Polymerase Chain Reaction commonly known as RT-PCR constitutes a reliable, fast and highly sensitive technique for finding viruses in specimens. This robust method normally uses biomarkers for the analysis of samples and presents increased specificity in the identification of viruses.

Employing real-time and non real-time RT-PCR, the newly developed technology is capable of detecting bovine enteroviruses (BEV) and porcine teschovirus (PTV). Both have been proven very accurate when used for testing animal faeces and water taken from areas located close to animal farms.

Not only was the technology capable of identifying BEV, but it could also distinguish between the various BEV genotypes within a number of species including cattle, while-tailed deer, goats, sheep and horses. Moreover, it presents increased potentialities for detecting residues of other animals with the aid of different enteric viruses that are specific for each species.

Compared to other conventional chemical analyses used, it is an equally high sensitive and specific technique for viral characterisation and differentiation of the animal origin species of the contaminants. This eco-friendly tool may be extremely useful in private and official laboratories that perform viral analyses for environmental and specifically water quality monitoring.
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