Community Research and Development Information Service - CORDIS

For next generation cryo-preservation applications

An innovative water-based breakthrough development enhances typical cryo-protective agents and media acting as a solvent for the cryo-preservation of cells and semen in Assisted Reproductive Technologies (ART) applications.
For next generation cryo-preservation applications
Cryo-protective agents are formed on the basis of molecules that prevent damage caused to tissues and cells by ice formation inside the cells. The most commonly used agents are glycerol and DMSO, which are considered to be genotoxic. Unlike these, the novel cryo-protecting technology is not only non-toxic to human sperm cells, but it also contributes to cell survivability after cryo-preservation.

The innovative cryo-protective agent is based on special nanotechnology that uses nanoparticles for introducing water-based biomaterials. More specifically, on the grounds of the unique properties of nanoparticles the technology is capable of modifying the physical properties of water molecules around them. Thereby, it produces a water structure featuring physical properties that mimic that of intracellular water through the usage of inorganic, insoluble crystals introduced in water in a patented process.

The technology was employed for the development of a water-based cryo-protectant for ART that enables optimisation of typical cryo-protective agents and media. By enhancing the binding of water molecules in solution, it prevents the formation of intracellular ice crystals as it replaces water molecules inside cells being frozen. The technology allows stabilisation of the cryo-preservation media during freeze and thaw.

The novel cryo-protective agent offers appropriate regulation of the oxido-reduction enzyme mechanism and reduces the harmful damages of free radicals, thereby protecting the inner mitochondrial cell membrane. The technology facilitates the maximisation of efficiency of superior water-based biocatalysts, solvents, reagents, media enhancers, and buffers in new and existing products and processes.

Collaborations are sought with manufacturers and providers of agent and media products related to the cryo-preservation of all types of biological samples. These include semen, serum, live cells, DNA, embryos, gametes and others.
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