Rapid detection of multiple pathogens
Pathogen detection is a laborious process that usually relies on microbiology-based methods. However, there are instances where information on contaminants is required immediately for appropriate containment measures to be taken. The EU-funded MULTISENSE CHIP (The lab-free CBRN detection device for the identification of biological pathogens on nucleic acid and immunological level as lab-on-a-chip system applying multisensor technologies) multidisciplinary project focused on two types of use. A one-time measurement system was developed as well as a permanent device that detects microbes for a longer time period. The Multisense Chip Analyser incorporates the complete process chain – from sample collection to target molecule extraction, followed by biochemical detection and finally, a readout process via different sensor technologies. Most importantly, this system will offer a lab-free approach without the need for specialist personnel and space. B priority pathogens including Brucella species and Bacillus anthracis were targeted in line with international biodefense and infectious diseases guidelines. Key to the development of this novel device is the use of advanced sensor technologies, lab-on-a-chip technology and innovative instrumentation. The detection is based on simultaneous analysis by polymerase chain reaction (PCR) and immunological methods, providing a fast response within the hour. For system realisation, partners performed extensive optimisation at the technical and biological levels. They have worked on the molecular assays, the sensor technologies, and the instrument development and set-up. Different ways of sampling were tested, alongside methods for extracting microbial DNA and amplifying specific regions of the genome by PCR. With respect to the immunoassay component of the chip, target molecules could be detected by electrochemiluminescent-, electrochemical- and fluorescence-based sensors. The multi-level analysis of biological pathogens will provide more accurate diagnosis and prove greatly beneficial for both medicine and veterinary science. The MULTISENSE CHIP system will also enable first responders and authorities to rapidly obtain information on potential contaminations and thus take appropriate measures.
Keywords
Detection, pathogen, MULTISENSE CHIP, lab-on-a-chip, sensor technologies, PCR, immunoassay
