Objective Recent developments in molecular tools combined with updated epidemiological data provide novel challenges to the development of modern molecular diagnostics. The increasing threat of infections due to Mycobacterium tuberculosis, including multidrug-resistant (MDR) and extensively drug-resistant (XDR) infections poses important questions that call for development of integrated tools for rapid diagnosis. In the specific case of tuberculosis (TB), an integrated rapid diagnostic approach should be able to allow at the same time species identification, drug susceptibility testing and molecular typing. We propose to develop and validate a silicon-based platform for molecular biology testing consisting in a single disposable device (biochip) and on associated specific instruments (reader). This technology is able to simultaneously serve as a platform for high specificity amplification and hybridisation of selected targets, and to provide the diagnostic report within few hours. Main advancement over existing technology (i.e. Real-Time PCR) consists in the possibility to perform the test at competitive costs, using an higher number of genetic probes by integrating multiple, separate PCR chambers and medium density array (50-200 probes), with faster and more stable amplification and hybridisation reactions through optimised and controlled thermal ramps and profile. In addition to the technology development, special focus will be put on the feasibility and cost-effectiveness of such a novel tool with high technological content for application in countries with high TB, MDR- and XDR-TB incidence. In parallel to the development and validation of the chip-based diagnostic platform for TB, a feasibility study on its applicability to other poverty related diseases such as malaria, will be carried out. In summary, the present proposal aims at developing an affordable, high-tech platform for rapid molecular diagnosis applicable to TB and other infectious and poverty-related diseases Fields of science medical and health scienceshealth sciencesinfectious diseasesmalariamedical and health sciencesclinical medicinepneumologytuberculosismedical and health sciencesbasic medicinepharmacology and pharmacydrug resistancemultidrug resistancenatural sciencesbiological sciencesmolecular biology Keywords MDR-TB XDR-TB biochips fast molecular diagnosis Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Topic(s) HEALTH-2007-2.3.2-3 - Development of fast tests for the diagnosis of Multi-Drug-Resistant strains of HIV, malaria and tuberculosis and of latent tuberculosis infection (LTBI) Call for proposal FP7-HEALTH-2007-A See other projects for this call Funding Scheme CP-FP - Small or medium-scale focused research project Coordinator FONDAZIONE CENTRO SAN RAFFAELE DEL MONTE TABOR EU contribution € 525 799,00 Address Via Olgettina 60 20132 MILANO Italy See on map Region Nord-Ovest Lombardia Milano Activity type Research Organisations Administrative Contact Maria Rosa Pedrazzi (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (8) Sort alphabetically Sort by EU Contribution Expand all Collapse all FORSCHUNGSZENTRUM BORSTEL LEIBNIZ LUNGENZENTRUM Germany EU contribution € 312 543,00 Address PARKALLEE 1-40 23845 Borstel See on map Region Schleswig-Holstein Schleswig-Holstein Segeberg Activity type Research Organisations Administrative Contact Susann Schrader (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITA DEGLI STUDI DI SIENA Italy EU contribution € 306 000,00 Address VIA BANCHI DI SOTTO 55 53100 Siena See on map Region Centro (IT) Toscana Siena Activity type Higher or Secondary Education Establishments Administrative Contact Silvano Focardi (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data NATIONAL CENTER OF INFECTIOUS AND PARASITIC DISEASES Bulgaria EU contribution € 90 000,00 Address blvd. Yanko Sakazov 26 1504 Sofia See on map Region Югозападна и Южна централна България Югозападен София (столица) Activity type Research Organisations Administrative Contact Bogdan Petrunov (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data STMICROELECTRONICS SRL Italy EU contribution € 284 077,00 Address VIA C.OLIVETTI 2 20864 Agrate Brianza See on map Region Nord-Ovest Lombardia Monza e della Brianza Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Maria Cristina Di Gesù (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data QENDRA SPITALORE RAJONALE E TIRANES SHEFQET NDROQI Albania EU contribution € 90 000,00 Address RRUGA DR SHEFQET NDROQI SAUK 1044 TIRANA See on map Region Shqipëria Qender Tiranë Activity type Higher or Secondary Education Establishments Administrative Contact Rezart Oketa (Dr.) Links Contact the organisation Opens in new window Total cost No data UNIVERSITY OF GLASGOW United Kingdom EU contribution € 389 373,00 Address UNIVERSITY AVENUE G12 8QQ Glasgow See on map Region Scotland West Central Scotland Glasgow City Activity type Higher or Secondary Education Establishments Administrative Contact Joseph Galloway (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data FOUNDATION FOR INNOVATIVE NEW DIAGNOSTICS Switzerland EU contribution € 300 000,00 Address CAMPUS BIOTECH, CHEMIN DES MINES 9 1202 Geneve See on map Region Schweiz/Suisse/Svizzera Région lémanique Genève Activity type Other Administrative Contact Herbert Clemens (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data QUEEN MARY UNIVERSITY OF LONDON United Kingdom EU contribution € 685 415,00 Address 327 MILE END ROAD E1 4NS London See on map Region London Inner London — East Tower Hamlets Activity type Higher or Secondary Education Establishments Administrative Contact Warren Lindsey (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data