Objective Chromatin-remodelling complexes (CRCs) play pivotal roles in the regulation of basic cellular processes, including transcription, DNA replication, repair and cell cycle in eukaryotes. Although several orthologues of CRC components are conserved between higher plants and other eukaryotes, our current knowledge on plant CRCs is rather limited. To unravel the regulatory functions of SWI/SNF-type CRCs, the proposed study focuses on functional analysis of SWI3 core subunits that are linked by ATPases and other interacting factors to specific signalling pathways. Recently, we demonstrated that SWI/SNF CRCs in Arabidopsis carry different combinations of SWI3 subunits that play distinct and essential roles in the control of cell differentiation, organ development, flowering time and gene silencing. The SWI3 subunits show unique interactions with several signalling factors, such as the flowering time regulator FCA. Through their SNF5/BSH CRC subunit, the CRC complexes interact with conserved components of stress signalling pathways, including the AMPK-activated protein kinases. The proposed training project is based on a focused multidisciplinary approach, which aims at the characterization of regulatory roles of SWI/SNF CRC complexes that carry ATSWI3C subunit. Complementary tasks of the experimental work plan include: i) the isolation and mass spectrometry analysis of CRC complexes carrying affinity tag labeled SWI3C subunit, ii) identification of genomic targets of SWI3C containing CRCs by chromatin cross-linking, iii) analysis of function of SWI3C-associated CRC components using reverse genetics, and iv) study of in vivo nuclear interaction of CRC subunits using fluorescence energy transfer-based confocal microscopy. The project is aided by expert mentoring activity of the host and is embedded in a carrier development plan envisioning long-term collaborative interaction with the researcher upon his return to the home institute. Fields of science natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesmathematicspure mathematicsmathematical analysisfunctional analysisnatural sciencesphysical sciencesopticsmicroscopyconfocal microscopynatural scienceschemical sciencesanalytical chemistrymass spectrometry Keywords FRET MALDI chromatin cross-linking chromatin remodelling regulation of gene expression stress signalling Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Call for proposal FP7-PEOPLE-2007-2-1-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV EU contribution € 166 982,27 Address HOFGARTENSTRASSE 8 80539 Munchen Germany See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Research Organisations Administrative Contact Csaba Koncz (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
