Cel
The attachment of Ubiquitin (Ub) to proteins (ubiquitination) is one of the most abundant post‐translational modifications in eukaryotes. Deregulation of the ubiquitination reaction is the cause of various diseases, including cancer. Ubiquitination is catalyzed by the sequential action of an activating (E1), a conjugating (E2), and a ligating (E3) enzyme. Ubiquitination enzymes contain catalytic cysteine residues that transfer Ub via thioester intermediates to a substrate lysine residue. The functional consequences of ubiquitination depend on the length and linkage of the attached Ub chain. While poly-ubiquitination commonly targets proteins for proteasomal degradation, mono- or multi-ubiquitination of receptor proteins serve as internalization or sorting signals. Interestingly, Ub ligases mediate both mono- and poly-ubiquitination depending on their substrates or other modulators. Although the basic principles of ubiquitination have been identified, the exact reaction mechanism underlying Ub protein ligation is still unknown. Furthermore, it is still unclear what determines whether a substrate is mono-, multi- or poly-ubiquitinated. To provide a structural basis of the ubiquitin protein ligation, we propose to use NMR spectroscopy in combination with biochemical tools to identify and characterize conformational changes and binding surfaces in HECT‐type Ub ligases at different steps of the ubiquitination reaction.
Dziedzina nauki
Zaproszenie do składania wniosków
FP7-PEOPLE-IRG-2008
Zobacz inne projekty w ramach tego zaproszenia
System finansowania
MC-IRG - International Re-integration Grants (IRG)Koordynator
80539 Munchen
Niemcy