Objective In the last years it has been shown that transplanted adult bone marrow derived stem cells (BMSCs) have the ability to fuse with cells of other types and restore several pathologies such as congenital liver failure and muscular dystrophy. On the nervous system, it was recently reported that bone marrow transplantation (BMT) made 24 hours after the stroke leads to a functional outcome. Cell fusion is one of the underlying mechanisms as it has been shown that it is indirectly implicated in the formation of vasculature after stroke. Thus, stroke is a pathology susceptible to be treated with this cell therapy mechanism. Despite the great potential of this mechanism of regeneration, the low frequency of fusion events, especially in the brain, has veiled and hindered the true regenerative potential of cell fusion. Therefore, the main objective of this project is to identify fusogenic factors that will increase cell fusion events to an effective level for cell therapy of neurological disorders. For this, firstly we will screen in vitro new putative fusogenic with the help of an in vitro cell fusion detection system and FACS analysis. Later, chitosan nanoparticles will be developed for the controlled release of the putative fusogenic factors. Finally, we will evaluate the in vivo efficacy of these factors trough a series of intravenous BMT co-administered with nanoparticles loaded with the selected factors in the mouse model of stroke. We will correlate an increment in the cell fusion events with the putative improvement in stroke symptoms. With this project we will improve our knowledge about adult stem cell plasticity and explore cell fusion process as a neuroregenerative process for the treatment of stroke. Fields of science medical and health sciencesbasic medicineneurologymuscular dystrophiesmedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesbasic medicinepathologymedical and health sciencesbasic medicineneurologystrokemedical and health sciencesclinical medicinetransplantation Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2009-IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Call for proposal FP7-PEOPLE-2009-IEF See other projects for this call Funding Scheme MC-IEF - Intra-European Fellowships (IEF) Coordinator AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS EU contribution € 152 917,00 Address CALLE SERRANO 117 28006 Madrid Spain See on map Region Comunidad de Madrid Comunidad de Madrid Madrid Activity type Research Organisations Administrative Contact Carlos Manuel Abad Ruiz (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
