Obiettivo Proper and controlled expression of genes is essential for normal cell growth. Chromatin modifying enzymes play afundamental role in the control of gene expression and their deregulation is often linked to cancer. In recent years chromatinmodifiers have been considered key targets for cancer therapy and this demands a full understanding of their biologicalfunctions. Previous biochemical and structural studies have focused on the identification of chromatin modifying enzymesand characterization of their substrate specificities and catalytic mechanisms. However, a comprehensive view of thebiological processes, signaling pathways and regulatory circuits in which these enzymes participate is missing. Proteinarginine methyltransferases (PRMTs), which methylate histones and are evolutionarily conserved from yeast to human,constitute an example of chromatin modifying enzymes whose functional and regulatory networks remain unexplored. Ipropose to use complementary state-of-the-art genomic and proteomic approaches in order to identify the protein networksand cellular pathways that are linked to PRMTs. In parallel, I will identify novel regulatory circuits and define the molecularmechanisms that control methylation of specific histone arginine residues. I will utilize the yeast S. cerevisiae as a modelorganism because it allows genetic, biochemical and genomic approaches to be combined. Most importantly, many of thepathways and mechanisms in yeast are highly conserved and therefore, the findings from this study will be pertinent tohuman and other eukaryotic organisms. Establishing a global cellular wiring diagram of PRMTs will serve as a paradigm forother chromatin modifiers and is imperative for assessing the efficacy of these enzymes as therapeutic targets. Campo scientifico natural sciencesbiological sciencesgeneticsmedical and health sciencesclinical medicineoncologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-SG-LS2 - ERC Starting Grant - Genetics,Genomics,Bioinformatics and Systems Biology Invito a presentare proposte ERC-2010-StG_20091118 Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-SG - ERC Starting Grant Istituzione ospitante UNIVERSITY OF CYPRUS Contributo UE € 1 498 278,80 Indirizzo AVENUE PANEPISTIMIOU 2109 AGLANTZI 1678 Nicosia Cipro Mostra sulla mappa Regione Κύπρος Κύπρος Κύπρος Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Xenia Constantinou (Ms.) Ricercatore principale Antonis Kirmizis (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto UNIVERSITY OF CYPRUS Cipro Contributo UE € 1 498 278,80 Indirizzo AVENUE PANEPISTIMIOU 2109 AGLANTZI 1678 Nicosia Mostra sulla mappa Regione Κύπρος Κύπρος Κύπρος Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Xenia Constantinou (Ms.) Ricercatore principale Antonis Kirmizis (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato