Objective Cilia and flagella are highly conserved cell organelles that exhibit regular, serpentine beating motions. Each cilium and flagellum contains a motile organelle called an axoneme, which is comprised of an array of microtubules, dynein motor proteins, and accessory proteins. Recent studies have led to the hypothesis that intrinsic mechanical and mechanochemical properties of the dyneins provide the feedback and coordination necessary for the regular beating pattern of cilia and flagella. The objective of this project is to test this mechanical coordination hypothesis. The proposal takes an interdisciplinary approach, bringing together experiments and theory and using analytical tools from informatics, biochemistry, biomechanics, and mechanical engineering. Specifically, the proposal aims to measure the motor properties of individual molecules and small ensembles of dyneins using optical tweezers. These measurements will be used to evaluate the state of the art models of axoneme beating, confirming or rejecting the dynein coordination hypotheses on which they are based. The measured motor properties of dynein will be used to build a new model of the axoneme from the “bottom-up.” The principle advantage of this model over the existing models is that, rather than showing that a set of theoretical molecular properties can fit experimental data on whole flagella, it will specifically account for the measured molecular details of the axoneme. The ability of the “bottom up” model to predict experimentally acquired flagellar beat patterns will be compared to models from the literature. This project will be part of an increasing number of studies on the role mechanics and forces have on biology at the cell level. It will not only further our understanding of dynein coordination and flagellar motion, but also biological coordination in general, a phenomenon that has allowed for the evolution of complex organisms, but has remained largely unexplained. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsengineering and technologymechanical engineeringnatural sciencesbiological sciencesbiophysics Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2010-IIF - Marie Curie Action: "International Incoming Fellowships" Call for proposal FP7-PEOPLE-2010-IIF See other projects for this call Funding Scheme MC-IIF - International Incoming Fellowships (IIF) Coordinator MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV EU contribution € 225 945,00 Address HOFGARTENSTRASSE 8 80539 Munchen Germany See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Research Organisations Administrative Contact Ruediger Hesse (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
