Objectif "Synthetic biology is still far from producing flexible, programmable, scalable and predictable engineered constructs able to perform complex computations. The main problem has arisen from the fact that, in contrast to electronic designs where wires have identical nature, in a cell-based system each wire would correspond to a different molecular entity. In a joint effort between theoretical and experimental studies, we have established biological circuits with Distributed Computation capacity (Regot et al., 2011), opening the possibility to develop a novel method of properly design general purpose, LEGO-like multicellular systems able to partially avoid wiring limitation. Here, we propose to explore the limits of Distributed Computation in synthetic cellular systems. To this goal, we will extend our previous circuits to circuits with a higher complexity, never achieved to date as well explore the use of different cellular systems as a biological case study. Our preliminary data indicate that there are two aspects, which were not considered previously, that when combined with Distributed Computation, could permit a strong reduction or even eliminate the wires in biological computation; the first aspect is the use of inverse logic circuits and the second is the development of spatially restricted devices. We will set up the theoretical framework of these novel aspects and establish in vivo circuits spatially restricted cellular systems. To this end we propose 1) to use microfluidic devices for physically restricted cellular networks and 2) to implement circuits with mixed but physiologically isolated cells (without wires) with different computation capabilities. In addition, we will extend these studies to obtain highly reprogrammable circuits. The combination of such approaches should demonstrate that biological computation is scalable, allows constructing arbitrary cell-based computing machines and breaks all current limitations concerning circuit complexity." Champ scientifique natural sciencesbiological sciencessynthetic biologyengineering and technologyelectrical engineering, electronic engineering, information engineeringinformation engineeringtelecommunicationstelecommunications networksmobile network Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-AG-ID1 - ERC Advanced Grant Interdisciplinary Panel Appel à propositions ERC-2011-ADG_20110310 Voir d’autres projets de cet appel Régime de financement ERC-AG - ERC Advanced Grant Institution d’accueil UNIVERSIDAD POMPEU FABRA Contribution de l’UE € 2 764 000,00 Adresse PLACA DE LA MERCE, 10-12 08002 Barcelona Espagne Voir sur la carte Région Este Cataluña Barcelona Type d’activité Higher or Secondary Education Establishments Chercheur principal Ricard Solé Vicente (Dr.) Contact administratif Eva Martín (Mrs.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire UNIVERSIDAD POMPEU FABRA Espagne Contribution de l’UE € 2 764 000,00 Adresse PLACA DE LA MERCE, 10-12 08002 Barcelona Voir sur la carte Région Este Cataluña Barcelona Type d’activité Higher or Secondary Education Establishments Chercheur principal Ricard Solé Vicente (Dr.) Contact administratif Eva Martín (Mrs.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée